Ajax Harwood Clinic
Lyme test (public health lab)
Full name: Lyme disease serology (two-tier, via Public Health Ontario)
This test is useful only in specific situations — it's not right for everyone who asks about it.
What this test is
This blood test checks for Lyme disease. Lyme disease is an infection spread by certain tick bites. The test looks for antibodies your body makes after exposure. If you have the classic early rash, a growing red circle where a tick bit you, your doctor can treat you right away. That's true if you also had a real chance of tick exposure. You would not need to wait for this test. The test is most useful without that rash. It helps when there's a real chance of tick exposure and symptoms that fit later-stage Lyme disease. Examples are joint swelling or nerve problems. Ontario's public health lab runs this test with a validated two-step method. It is not a good way to explain ongoing fatigue or vague symptoms on its own. You need a tick exposure history to go with it.
Want to see the full clinical reasoning? The reference we use for this test is open to anyone — it is detailed and written for clinicians, and you are welcome to read it.
Open the clinician reference →Looking for other tests, or more on a condition an ND raised? Explore ask.ajaxharwoodclinic.com →
General health information for Ajax Harwood Clinic patients and the public — not medical advice, and not a substitute for seeing your own doctor. Last reviewed 2026-09-23.